recombinant rat il 1β cytokine  (R&D Systems)

Journal: Frontiers in Immunology

Article Title: FLT3LG and IFITM3P6 consolidate T cell activity in the bone marrow microenvironment and are prognostic factors in acute myelocytic leukemia

doi: 10.3389/fimmu.2022.980911

Figure Lengend Snippet: Screening of survival-related differentially expressed genes. (A) The intersection of candidate genes in TCGA and TARGET datasets. (B) Univariate cox regression analysis of 6 survival-related genes in TCGA. (C) Multivariate cox regression analysis included RP11-23J18.1 and FLT3LG as survival predicting gene models in TCGA. (D, E) Application of the survival predicting model in TCGA dataset.

Article Snippet: Added 1μg/ml, 10μg/ml, 50μg/ml, 100μg/ml and 200μg/ml of recombinant rat Flt3lg cytokine (PEPROTECH, USA) per well (6-well plate) to stimulate the activation of dendritic cells and performed qRT-PCR, found that expression of CD40 was higher than the negative control, and its expression presented positive dose-dependent ( ).

Techniques:

Journal: Frontiers in Immunology

Article Title: FLT3LG and IFITM3P6 consolidate T cell activity in the bone marrow microenvironment and are prognostic factors in acute myelocytic leukemia

doi: 10.3389/fimmu.2022.980911

Figure Lengend Snippet: Evaluation of tumor microenvironment (TME). (A) Heatmap of immune cell, stromal cell and ESTIMATE scores and tumor purity in high and low immune activity groups. (B–E) Relationship between immune cell, stromal cell and ESTIMATE scores and tumor purity with immune activity in the TME. (F, G) Relationship between FLT3LG and RP11-23J18.1 expression and immune activity. *** p < 0.001.

Article Snippet: Added 1μg/ml, 10μg/ml, 50μg/ml, 100μg/ml and 200μg/ml of recombinant rat Flt3lg cytokine (PEPROTECH, USA) per well (6-well plate) to stimulate the activation of dendritic cells and performed qRT-PCR, found that expression of CD40 was higher than the negative control, and its expression presented positive dose-dependent ( ).

Techniques: Activity Assay, Expressing

Journal: Frontiers in Immunology

Article Title: FLT3LG and IFITM3P6 consolidate T cell activity in the bone marrow microenvironment and are prognostic factors in acute myelocytic leukemia

doi: 10.3389/fimmu.2022.980911

Figure Lengend Snippet: GO term enrichment analysis of FLT3LG and RP11_23J18.1 by GSEA method. (A) GO analysis of FLT3LG. (B) GO analysis of RP11_23J18.1.

Article Snippet: Added 1μg/ml, 10μg/ml, 50μg/ml, 100μg/ml and 200μg/ml of recombinant rat Flt3lg cytokine (PEPROTECH, USA) per well (6-well plate) to stimulate the activation of dendritic cells and performed qRT-PCR, found that expression of CD40 was higher than the negative control, and its expression presented positive dose-dependent ( ).

Techniques:

Journal: Frontiers in Immunology

Article Title: FLT3LG and IFITM3P6 consolidate T cell activity in the bone marrow microenvironment and are prognostic factors in acute myelocytic leukemia

doi: 10.3389/fimmu.2022.980911

Figure Lengend Snippet: Single-cell transcriptome analysis revealed detailed gene location in various cell types. (A, B) UMAP cluster after cluster analysis of peripheral blood mononuclear cells (PBMC) and acute myelocytic leukemia (AML). (C, E) Violin plot and heatmap of FLT3LG and FLT3 expression in the PBMC_30K_10× dataset. (D, F) The transcription level of FLT3LG and FLT3 in cell types of the AML_GSE116256 dataset. (G) FLT3LG expression was positively correlated with CD40 expression in TCGA data. (H) CD40 expression was significantly elevated when Flt3lg cytokine activation in dendritic cells. *** p < 0.001; **** p < 0.0001.

Article Snippet: Added 1μg/ml, 10μg/ml, 50μg/ml, 100μg/ml and 200μg/ml of recombinant rat Flt3lg cytokine (PEPROTECH, USA) per well (6-well plate) to stimulate the activation of dendritic cells and performed qRT-PCR, found that expression of CD40 was higher than the negative control, and its expression presented positive dose-dependent ( ).

Techniques: Expressing, Activation Assay

Journal: Frontiers in Immunology

Article Title: FLT3LG and IFITM3P6 consolidate T cell activity in the bone marrow microenvironment and are prognostic factors in acute myelocytic leukemia

doi: 10.3389/fimmu.2022.980911

Figure Lengend Snippet: Functional mechanism analysis for FLT3LG, IFITM3P6 and CBX7. (A, B) Relationship between FLT3 DNA methylation and FLT3 expression and the main methylation site. (C–G) Relationship between FLT3LG expression and N 6 -methyladenosine (m6A) methylation-related gene expression. (H) Relationship between IFITM3P6 expression and m6A methylation-related gene expression. (I–M) Relationship between CBX7 expression and m6A methylation-related gene expression.

Article Snippet: Added 1μg/ml, 10μg/ml, 50μg/ml, 100μg/ml and 200μg/ml of recombinant rat Flt3lg cytokine (PEPROTECH, USA) per well (6-well plate) to stimulate the activation of dendritic cells and performed qRT-PCR, found that expression of CD40 was higher than the negative control, and its expression presented positive dose-dependent ( ).

Techniques: Functional Assay, DNA Methylation Assay, Expressing, Methylation, Gene Expression

Journal: Frontiers in Immunology

Article Title: FLT3LG and IFITM3P6 consolidate T cell activity in the bone marrow microenvironment and are prognostic factors in acute myelocytic leukemia

doi: 10.3389/fimmu.2022.980911

Figure Lengend Snippet: The potential relationship of FLT3LG and IFITM3P6 in activating T cells. (A) FLT3LG is significantly correlated with IFITM3P6 in TCGA data. (B) FLT3LG is positively correlated with CBX7. (C, D) FLT3LG and IFITM3P6 are enriched in NOTCH signaling pathway. (E, F) CBX7 is positively correlated with NOTCH1 and MAML2. (G) Dll1 is increased in rat bone marrow-derived dendritic cells along with elevated concentration (μg/ml) of Flt3lg stimulation. * p < 0.05; ** p < 0.01; ns, no significance.

Article Snippet: Added 1μg/ml, 10μg/ml, 50μg/ml, 100μg/ml and 200μg/ml of recombinant rat Flt3lg cytokine (PEPROTECH, USA) per well (6-well plate) to stimulate the activation of dendritic cells and performed qRT-PCR, found that expression of CD40 was higher than the negative control, and its expression presented positive dose-dependent ( ).

Techniques: Derivative Assay, Concentration Assay

Journal: Frontiers in Immunology

Article Title: FLT3LG and IFITM3P6 consolidate T cell activity in the bone marrow microenvironment and are prognostic factors in acute myelocytic leukemia

doi: 10.3389/fimmu.2022.980911

Figure Lengend Snippet: The main mechanisms of FLT3LG and IFITM3P6 in T cell activation. On the one hand, increased DLL1 by stimulation of FLT3LG to FLT3 in dendritic cells, transduct signals to NOTCH1 receptor expressed by T cells. Ligand/receptor binding triggers sequential proteolytic cleavage of NOTCH receptor, first by the ADAM10 metalloprotease and by the γ-secretase complex. These cleavages release intracellular NOTCH (ICN) into the nucleus. On the other hand, IFITM3P6 as a kind of ceRNA inhibit targeted degradation of CBX7 by miR-6748-3p. They finally promote the formation of NOTCH1-MAML2-CBX7 complex system, and suppress expression of FasL.

Article Snippet: Added 1μg/ml, 10μg/ml, 50μg/ml, 100μg/ml and 200μg/ml of recombinant rat Flt3lg cytokine (PEPROTECH, USA) per well (6-well plate) to stimulate the activation of dendritic cells and performed qRT-PCR, found that expression of CD40 was higher than the negative control, and its expression presented positive dose-dependent ( ).

Techniques: Activation Assay, Binding Assay, Expressing